Recombinant:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
Reverse Transcription:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
SYBR Green Assay:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
SDS Page:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
Membrane:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
Western Blot:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
Bicinchoninic Acid Protein Assay:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
Staining:Article Title: Cold-induced thermogenesis requires neutral-lipase-mediated intracellular lipolysis in brown adipocytes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal ATGL Cell Signaling Technology Cat# 2138, lot 6; RRID:AB_2167955 Rabbit monoclonal HSL Cell Signaling Technology Cat# 18381, clone D6W5S, lot 1; RRID:AB_2798800 Rabbit monoclonal GAPDH Cell Signaling Technology Cat# 2118, clone 14C10, lot 14; RRID:AB_561053 Anti-rabbit horse radish peroxidase-labelled secondary antibody Cell Signaling Technology Cat# 7074, lot 32 CD45-VioBlue REAfinity, Miltenyi 130-110-802; RRID:AB_2658222 F4/80 APC REAfinity, Miltenyi 130-116-525; RRID:AB_2733417 CD31-PE REAfinity, Miltenyi 130-111-540 Rat anti-mouse CD16/32 BD Biosciences Cat# 553141, clone 2.4G2; RRID:AB_394656 Chemicals, peptides, and recombinant proteins CL-316,243 Sigma-Aldrich Cat# C5976 Tamoxifen Free Base Sigma-Aldrich Cat# T5648 Multiscribe Reverse Transcriptase ThermoFischer Scientific Cat# 4368813 Fast SYBR Green Master Mix ThermoFischer Scientific Cat# 4385614 Taqman Fast Advanced Master Mix ThermoFischer Scientific Cat# 4444558 Clostridium histolyticum collagenase Sigma C6885 LiberaseTM Research Grade Roche 05401127001 Streptomyces hyalurolyticus hyaluronydase Sigma H1136 SDS-PAGE gel (4-20% gradient) Biorad Cat# 5678094 ou 5678095 Trans-Blot turbo RTA transfert kit, nitrocellulose membrane Biorad Cat# 1704271 Clarity Western ECL Substrate Biorad Cat# 170-5060 Aspergillus niger amyloglucosidase Sigma Cat# 10113-1G, lot BCBD1453V D-(+) glucose Sigma G8270 Bovine serum albumin Sigma A7030 Formalin solution Sigma HT501128 Eosin G o Y 0.5% alcoholic Diapath C0353 Mayer hematoxylin Diapath C0303 Cacodylate buffer EMS 11655 EMbed812 resin EMS 14120 Critical commercial assays NEFA C kit Fujifilm Wako Cat# SBDW1W434-91795; SBDW1W436-91995 Free Glycerol reagent Sigma Cat# F6428 Glucose GOD FS kit DiaSys Cat# 1 2500 99 10 026, lot 24781 Insulin ALPCO Diagnostics Cat# 80-INSMS-E1 Ketone bodies Abbott Cat# 71302-75 Qiazol buffer Qiagen Cat# 79306 RNeasy kit Qiagen Cat# 74106 DNaseI Invitrogen Cat# 18068-015 BCA Protein Assay kit ThermoFisher Cat #23225 Zombie UV BioLegend 423107 Brilliant Stain Buffer BD Biosciences 563794 Experimental models: Organisms/strains C57BL/6J Janvier Laboratories N/A (Continued on next page) Cell Metabolism 37, 1–12.e1–e5, February 4, 2025 e1 .. C57BL/6J mice were obtained from Janvier Laboratories.
|
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
